High Pressure Gradient Mixing HPLC
Easy to use, Versatile Modular HPLC
Whatever your HPLC requirements, Adept HPLC will provide great value plus more.
Because the systems are modular, different detectors may be accommodated depending on analytical requirements. These detectors include variable wavelength UV/Visible, WaveQuest UV/Visible ultra-
If required, extra pumps such as semi-
A wide range of autosamplers, fraction collectors, column heater/chillers, column reactors and column switching valves may be included in an Adept HPLC system.
Each Adept system can be customised to an analytical requirement.
The use of post-
The use of biocompatible options brings a streamlined approach to denaturing DNA, semi-
Analyse catecholamines in blood plasma with an electrochemical detector, whilst simultaneously analysing anions using a conductivity detector.
Detect all organic acids in wine using the conductivity detector, whist simultaneously detecting the sugars with refractive index or electrochemical detectors.
High Pressure Mixing Gradients
Using the pulse-
This makes method development and method transfer reliable and less stressful.
Comprehensive, Versatile, Intuitive and Easy to Use PowerStream software
All this provides for easy operation. PowerStream Software also provides 21 CFR part 11 Compliance.
Chromatogram of some common amino acids
PowerStream – System Manager Chromatography Software
PowerStream is a chromatography management system developed by Cecil Instruments. Comprehensive data processing and full control of Cecil HPLC modules is provided. PowerStream complies with the terms of 21 CFR part 11.
PowerStream, developed to operate in Windows facilitates organisation of chromatography results and methods and provides digital control for a wide range of peripherals.
Operation is remarkably easy and help screens provide a complete operating guide.
PowerStream is easy to use, with a fully interactive graphical interface and display providing full method development with customised parameters, data capture and manipulation of data and chromatograms. A tutorial is included for clarity and rapid learning.
The complete range of chromatographic applications may be undertaken. Pumps may be fully programmed for any required gradient and the gradient profile may be overlayed on stored chromatograms for accurate mixture or flow adjustments. Mid elution wavelength control of the programmable UV detector is provided. Calibration curves, using any number of standards, may be computed and plotted for each component of interest and may subsequently be used to determine the concentration of unknown samples.
Comprehensive integration parameters are provided for accurate peak area and height calculations. Retention times for all peaks are tabulated.
The clarity of screen presentation is illustrated by the composite display shown for a typical gradient elution.
Clear Screen Presentation
Method creation is simple and convenient. Firstly select all the hardware to be used for the experiment and add any of the following parameters as required: Timed events, Integration parameters, Calibration curves and Reporting parameters.
PowerStream Interface, CE 4900
PowerStream is the powerful new chromatography software, developed by Cecil Instruments R & D, for HPLC system control and data processing. It is used in conjunction with DataStream software, also developed by Cecil Instruments, to provide data transfer and comprehensive display, processing and manipulation of spectral scans.
The PowerStream system manager interface unit, shown here, is designed as a space saving stackable module. The unit is supplied whenever
CE 4900/CE 4900D PowerStream system control and data processing are required.
When system control is not required, PowerStream CE 4901 is provided, with an interface module without the control feature.
- Personal Computer with Pentium class processor.
- Pentium 1GHz or higher.
- Microsoft Windows XP, Vista, 7, 8, 10.
- 512 MB of RAM or more.
disk space required: 150 MB.
- Monitor resolution 1024 x 768 or better.
- Microsoft Mouse or compatible pointing device.
- USB port.
HPLC Pump CE 4100
0.001 to 10mL/min in 0.001 steps
Better than 0.1%
40MPa = 400 bar
4 line back-
Full range of keys for menu and parameter selection
Fast number entry keys
Over Pressure Cut-
Adjustable between 3 and 40MPa
Low Pressure Cut-
Adjustable between 0 and 40MPa
Material in contact with fluids
Ceramic inserts –
Check valves – ruby and sapphire
Less than ±0.5% at 2mL/min
Size and Weight
365 x 290 x 140mm, 7.5kg.
115V or 230V, 50/60Hz, 30VA
Quaternary Low Pressure Pump CE 4104
0.001 to 10mL/min in 0.001/min increments
<±0.5% at 2mL/min
Quaternary to Binary low pressure mixing and isocratic high pressure operation
Using proprietary high-
Typically 0.2% RSD at 1mL/min
Material in contact with fluids
Ceramic inserts, ruby and sapphire check valves, PEEK
Binary gradient low pressure mixing and isocratic high pressure operation
as for the CE 4104
Binary Low Pressure Pump CE 4102
±0.35 x 10-
±0.3 x 10-
Dual Wavelength Operation
Simultaneous measurements at 2 wavelengths
Output of Absorbance ratio for the 2 wavelengths
Noise, Dual Wavelength
± 2 x 10-
Drift, Dual Wavelength
± 1 x 10-
Programs may be entered to change parameters, operating modes and select up to 6 different wavelengths in any time sequence.
Parallel printer port
Dual Wavelength, Programmable UV Visible Detector CE 4200
Double beam with 1200 lines/mm grating monochromator
± 0.1nm, single wavelength mode
< .02%, 220nm
Deuterium lamp with elapsed time indicator
0.001 to 2A full scale in 11 ranges
0.1, 0.2, 0.5, 1, 2, 5 and 10 seconds
Entry By use of special number keys
From keypad or by external source
For up to 30 methods, security protected
Second Order Filter
Fast automatic insertion at 370nm
± 1 x 10-
± 1 x 10-
Better than 1%, 0.001 – 2A
Sample Flow Cell
8µL x 10mm pathlength – normal
4µL x 5mm pathlength
Wetted surfaces PEEK, Teflon and Fused Silica
Other cells available
Refractive Index Sensitivity
< ± 5 x 10-
Flow Rate Sensitivity
< ± 3.5 x 10-
Two Absorbance outputs and Absorbance ratio to recorder. All data available to PowerStream System Manager.
Size and Weight
365 x 290 x 140mm, 17kg
115V or 230V, 50/60Hz, 50VA
With 1200 l/mm holographic grating
4nm – constant
0 to 3A
Up to 1000nm/s
± 0.002A over most of range
<0.02% at 220nm and 340nm
< ±0.5 x 10–5 A at 230nm, 2s air test cell
< ±0.5 x 10–4 A at 230nm, using air test cell
Calibration and Test
Automatic at switch-
Four line display of parameters, menus, prompts, wavelength, Absorbance etc.
Number of Peak Scans
Up to 100 peaks for any single elution
Multiple Scans of a Peak
Up to 8 scans of any peak
At start, set Absorbance value, point of inflection or at maximum
Up to 100, password protected, unlimited with PowerStream
Up to 30 methods, password protected
365 x 290 x 140mm, 17Kg
115V or 250V, 50/60Hz, 50VA
WaveQuest D.A.D. Ultra Fast Scanning Detector CE 4300
8 x 10-
8 x 10-
0.1, 0.2, 0.5, 1, 2, 5, 10 seconds
Size and weight
360 x 140 x 260mm, 9.5kg
Refractive Index Detector CE 4700
10W Xenon flash lamp, frequency programmable
Excitation and emission independently programmable
S/N >3000 for 4µg/L Anthracene in Methanol
10mV, 100mV and 1V
Fluorescence Detector Dual Monochromator CE 4500
Conductance Display Range
0.01 – 5000µS. cm-
0.1, 0.2, 0.5, 1, 1.5, 2, 5s
Typically up to 10,000µS. cm-
Synchronous Detection Frequency
Less than ±1nS/cm, DI water, 1mL/min, 2s
Less than ±10nS/cm/h, DI water, 1mL/min,
PEEK P1000 grade
316 stainless steel
Cell Pressure Rating
25 – 50ºC; 35ºC standard
10 minutes typical
Up to 100 with password protection
By PowerStream software
Conductivity Detector CE 4710
5ºC to 70ºC (maximum 12ºC below ambient)
Temperature and Set Point Readout
3 digit, bright LED display
Temperature Readout Resolution
Number of Columns
3 up to 25cm long with pre-
30 mins to 60ºC set point (ambient 25ºC)
Negligible when set point reached
Vapour detector – adjustable shut down level
50 Watts, 100-
Size and Weight
390H x 118W x 220D 6.4kg.
Cooled and Heated Oven CE 4600
Number of flow channels
4 (CE4040), 2 (CE 4020)
Materials Contacting Solvents
PEEK, glass filled PTFE, Teflon AF
To better than 1ppm at 1mL/min
Solvent Degassers CE 4020 / CE 4040
Headspace pressure, using dual concentric needle with internal air pump
100 vial carousel
2 mL, 32 x 12 mm diameter
Replicate injections per vial
Up to 15 (dependent on loop size)
Random access sampling
Injection volume reproducibility
Better than 0.2%, (typically <0.15%)
Better than 0.005% with active needle wash
Better than 0.03% with no needle wash
Sample loop flush
Wash station capacity
Dynamic wash system
Press buttons and backlit LCD for data menu and instructions
PC control using Cecil PowerStream software
RS232 and digital
Ambient temperature range
10°C to 35° C
36 x 40 x 33 cm (l x d x h)
AutoQuest Autosampler 4800-
Cecil Instruments policy is one of continuous development. We therefore reserve the right to change specifications without notice.