HPLC CECIL INSTRUMENT SYSTEM UK

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HPLC CECIL INSTRUMENT SYSTEM UK Reviewed by on . This Is Article About HPLC CECIL INSTRUMENT SYSTEM UK

High Pressure Gradient Mixing HPLC Easy to use, Versatile Modular HPLC Whatever your HPLC requirements, Adept HPLC will provide great value plus more. Because the systems are modular, different detectors may be accommodated depending on analytical requirements.  These detectors include variable wavelength UV/Visible, WaveQuest UV/Visible ultra-fast scanning, electrochemical, refractive index,… Selengkapnya »

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26-09-2018
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High Pressure Gradient Mixing HPLC

Easy to use, Versatile Modular HPLC

Whatever your HPLC requirements, Adept HPLC will provide great value plus more.

Because the systems are modular, different detectors may be accommodated depending on analytical requirements.  These detectors include variable wavelength UV/Visible, WaveQuest UV/Visible ultra-fast scanning, electrochemical, refractive index, conductivity and fluorescence.  Each detector is of the highest industry specification and is renowned for its Ultra-Low Drift.

If required, extra pumps such as semi-preparative and biocompatible,  may be acquired at any stage.

A wide range of autosamplers, fraction collectors, column heater/chillers, column reactors and column switching valves may be included in an Adept HPLC system.

Each Adept system can be customised to an analytical requirement.

The use of post-column reactors for amino acids, aflatoxins and barbiturates is easily achieved.

The use of biocompatible options brings a streamlined approach to denaturing DNA, semi-preparative proteomics HPLC etc.

Analyse catecholamines in blood plasma with an electrochemical detector, whilst simultaneously analysing anions using a conductivity detector.

Detect all organic acids in wine using the conductivity detector, whist simultaneously detecting the sugars with refractive index or electrochemical detectors.

High Pressure Mixing Gradients

Using the pulse-free microprocessor controlled pumps, achieve precise control and repeatability of mobile phase mixtures, to 0.1% levels.  Coupled with rapid responses to changes in concentration and lower incidences of bubbling of the mixed mobile phase, the achieved gradients are the best.

This makes method development and method transfer reliable and less stressful.

Comprehensive, Versatile, Intuitive and Easy to Use PowerStream software

All this provides for easy operation.  PowerStream Software also provides 21 CFR part 11 Compliance.

Chromatogram of some common amino acids

PowerStream – System Manager Chromatography Software

PowerStream is a chromatography management system developed by Cecil Instruments. Comprehensive data processing and full control of Cecil HPLC modules is provided.  PowerStream complies with the terms of 21 CFR part 11.

PowerStream, developed to operate in Windows  facilitates organisation of chromatography results and methods and provides digital control for a wide range of peripherals.

Operation is remarkably easy and help screens provide a complete operating guide.

PowerStream is easy to use, with a fully interactive graphical interface and display providing full method development with customised parameters, data capture and manipulation of data and chromatograms. A tutorial is included for clarity and rapid learning.

Complete Flexibility

The complete range of chromatographic applications may be undertaken. Pumps may be fully programmed for any required gradient and the gradient profile may be overlayed on stored chromatograms for accurate mixture or flow adjustments. Mid elution wavelength control of the programmable UV detector is provided. Calibration curves, using any number of standards, may be computed and plotted for each component of interest and may subsequently be used to determine the concentration of unknown samples.

Comprehensive integration parameters are provided for accurate peak area and height calculations. Retention times for all peaks are tabulated.

Convenient Operation

The clarity of screen presentation is illustrated by the composite display shown for a typical gradient elution.

Clear Screen Presentation

Method creation is simple and convenient. Firstly select all the hardware to be used for the experiment and add any of the following parameters as required: Timed events, Integration parameters, Calibration curves and Reporting parameters.

Method Operation

          

PowerStream Interface, CE 4900

        

PowerStream is the powerful new chromatography software, developed by Cecil Instruments R & D, for HPLC system control and data processing. It is used in conjunction with DataStream software, also developed by Cecil Instruments, to provide data transfer and comprehensive display, processing and manipulation of spectral scans.

The PowerStream system manager interface unit, shown here, is designed as a space saving stackable module. The unit is supplied whenever

CE 4900/CE 4900D PowerStream system control and data processing are required.

 When system control is not required, PowerStream CE 4901 is provided, with an interface module without the control feature.

 

 

System Requirements:-

  • Personal Computer with Pentium class processor.
  • Pentium 1GHz or higher.
  • Microsoft Windows XP, Vista, 7, 8, 10.
  • 512 MB of RAM or more.
  • Hard-disk space required: 150 MB.
  • CD-ROM drive.
  • Monitor resolution 1024 x 768 or better.
  • Microsoft Mouse or compatible pointing device.
  • USB port.

HPLC Pump CE 4100

Flow Rate

0.001 to 10mL/min in 0.001 steps

Flow Reproducibility

Better than 0.1%

Maximum Pressure

40MPa = 400 bar

User Interface

4 line back-lit display

Full range of keys for menu and parameter selection

Fast number entry keys

Over Pressure Cut-out

Adjustable between 3 and 40MPa

Low Pressure Cut-out

Adjustable between 0 and 40MPa

Material in contact with fluids

Ceramic inserts – biocompatible

Check valves – ruby and sapphire

Pulsation

Less than ±0.5% at 2mL/min

Size and Weight

365 x 290 x 140mm, 7.5kg.

Power Requirements

115V or 230V, 50/60Hz, 30VA

Quaternary Low Pressure Pump CE 4104

Flow Rate

0.001 to 10mL/min in 0.001/min increments

Pressure Range

0-40MPa over flow range

Flow Accuracy

<±0.5%

Flow Precision

<0.1% RSD

Pressure Pulsation

<±0.5% at 2mL/min

Gradient Forming

Quaternary to Binary low pressure mixing and isocratic high pressure operation

Gradient Control

Using proprietary high-speed proportioning valves

Gradient Precision

Typically 0.2% RSD at 1mL/min

Material in contact with fluids

Ceramic inserts, ruby and sapphire check valves, PEEK

Gradient Forming

Binary gradient low pressure mixing and isocratic high pressure operation

Other specifications

as for the CE 4104

Binary Low Pressure Pump CE 4102

Noise

±0.35 x 10-5 A at 230nm, 2s, using air test cell

Drift

±0.3 x 10-4 A at 230nm, 2s, using air test cell

Dual Wavelength Operation

Simultaneous measurements at 2 wavelengths

Output of Absorbance ratio for the 2 wavelengths

Noise, Dual Wavelength

± 2 x 10-5 A peak to peak 230/280nm 1s time constant

Drift, Dual Wavelength

± 1 x 10-4 A/hour at constant temperature

Programmed Operation

Programs may be entered to change parameters, operating modes and select up to 6 different wavelengths in any time sequence.

Printer Output

Parallel printer port

Dual Wavelength, Programmable UV Visible Detector CE 4200

Optical System

Double beam with 1200 lines/mm grating monochromator

Wavelength Range

190-700nm

Wavelength Accuracy

± 1nm

Wavelength Precision

± 0.1nm, single wavelength mode

Optical Bandwidth

8nm

Straylight

< .02%, 220nm

Lamp

Deuterium lamp with elapsed time indicator

Absorbance Range

0.001 to 2A full scale in 11 ranges

Time Constant

0.1, 0.2, 0.5, 1, 2, 5 and 10 seconds

Display

Four-line display of parameters, menus, prompts, wavelength, Absorbance etc.

Fast Number

Entry By use of special number keys

Event Marking

From keypad or by external source

Method Storage

For up to 30 methods, security protected

Second Order Filter

Fast automatic insertion at 370nm

Noise

± 1 x 10-5 A peak to peak, 220nm, 2s time constant

Drift

± 1 x 10-4 A/hour at constant temperature

Linearity

Better than 1%, 0.001 – 2A

Automatic Power-up/off

Pre-programmed from keypad to save warm up time

Sample Flow Cell

8µL x 10mm pathlength – normal

4µL x 5mm pathlength

Wetted surfaces PEEK, Teflon and Fused Silica

Other cells available

Refractive Index Sensitivity

< ± 5 x 10-4 A, water to methanol at 350nm

Flow Rate Sensitivity

< ± 3.5 x 10-3 A, for flow change 1-2mL/min, 300nm

Outputs

Two Absorbance outputs and Absorbance ratio to recorder. All data available to PowerStream System Manager.

Inputs

Event, autozero

Size and Weight

365 x 290 x 140mm, 17kg

Power Requirements

115V or 230V, 50/60Hz, 50VA

UV-Visible Detector CE 4201

Optical System

Double beam

Monochromator

With 1200 l/mm holographic grating

Wavelength Range

190-700nm

Optical Bandwidth

4nm – constant

Digital Resolution

1nm

Wavelength Accuracy

1nm

Wavelength Reproducibility

0.1nm

Absorbance Range

0 to 3A

Scan Speeds

Up to 1000nm/s

Baseline Flatness

± 0.002A over most of range

Straylight

<0.02% at 220nm and 340nm

Noise

< ±0.5 x 10–5 A at 230nm, 2s air test cell

Drift

< ±0.5 x 10–4 A at 230nm, using air test cell

Calibration and Test

Automatic at switch-on

Display

Four line display of parameters, menus, prompts, wavelength, Absorbance etc.

Number of Peak Scans

Up to 100 peaks for any single elution

Multiple Scans of a Peak

Up to 8 scans of any peak

Scan Trigger

At start, set Absorbance value, point of inflection or at maximum

Scan Storage

Up to 100, password protected, unlimited with PowerStream

Method Storage

Up to 30 methods, password protected

Size

365 x 290 x 140mm, 17Kg

Power Requirement

115V or 250V, 50/60Hz, 50VA

WaveQuest D.A.D. Ultra Fast Scanning Detector  CE 4300

Measuring Ranges

± 10-RIU

Sensitivity

8 x 10-RIU

Noise

8 x 10-RIU

Time constants

0.1, 0.2, 0.5, 1, 2, 5, 10 seconds

Cell volume

9µL

Outputs

1V to +1V, 20 bit resolution

Size and weight

360 x 140 x 260mm, 9.5kg

Power requirements

100-250V, 50/60Hz, 6VA

Refractive Index Detector CE 4700

Light Source

10W Xenon flash lamp, frequency programmable

Detector

Photomultiplier

Excitation Monochromator

200-650nm

Emission Monochromator

200-900nm, with extended range photomultiplier

Wavelength Scanning

Excitation and emission independently programmable

Sensitivity

S/N >3000 for 4µg/L Anthracene in Methanol

Analogue Outputs

10mV, 100mV and 1V

Power Requirements

100/240V, 50/60Hz

Fluorescence Detector Dual Monochromator CE 4500

Conductance Display Range

0.01 – 5000µS. cm-1

Time Constant

0.1, 0.2, 0.5, 1, 1.5, 2, 5s

Autozero Range

Typically up to 10,000µS. cm-1

Synchronous Detection Frequency

5KHz

Noise

Less than ±1nS/cm, DI water, 1mL/min, 2s

Drift

Less than ±10nS/cm/h, DI water, 1mL/min,

Cell Volume

0.5µL

Cell Body

PEEK P1000 grade

Electrodes

316 stainless steel

Cell Pressure Rating

200psi

Operating Temperature

25 – 50ºC; 35ºC standard

Temperature Precision

± 0.01ºC

Warm-up Time

10 minutes typical

Method Storage

Up to 100 with password protection

Outputs

0-1V analogue and RS232

Digital Control

By PowerStream software

Conductivity Detector CE 4710

Temperature Range

5ºC to 70ºC (maximum 12ºC below ambient)

Temperature and Set Point Readout

3 digit, bright LED display

Temperature Readout Resolution

0.1ºC

Temperature Stability

± 0.01ºC

Number of Columns

3 up to 25cm long with pre-columns accommodated

Heating Rate

30 mins to 60ºC set point (ambient 25ºC)

Stabilising Time

Negligible when set point reached

Leak Detection

Vapour detector – adjustable shut down level

Power Requirement

50 Watts, 100-240 V, 50/60Hz.

Size and Weight

390H x 118W x 220D 6.4kg.

Cooled and Heated Oven CE 4600

Number of flow channels

4 (CE4040), 2 (CE 4020)

Maximum Flowrate

10mL/min

Materials Contacting Solvents

PEEK, glass filled PTFE, Teflon AF

Gas removal

To better than 1ppm at 1mL/min

Solvent Degassers CE 4020 / CE 4040

Sampling system

Headspace pressure, using dual concentric needle with internal air pump

Capacity

100 vial carousel

Injection vial

2 mL, 32 x 12 mm diameter

Loop size

10-200 µL, 20 µL standard

Replicate injections per vial

Up to 15 (dependent on loop size)

Random access sampling

Yes

Priority sampling

Yes

Injection volume reproducibility

Better than 0.2%, (typically <0.15%)

Carryover

Better than 0.005% with active needle wash

Better than 0.03% with no needle wash

Sample loop flush

Operator selectable

Wash station capacity

12 mL

Needle wash

Dynamic wash system

Injection valve

Rheodyne

Local control

Press buttons and backlit LCD for data menu and instructions

Remote control

PC control using Cecil PowerStream software

Communications

RS232 and digital

Ambient temperature range

10°C to 35° C

Power requirements

110-250V, 50/60Hz

Dimensions

36 x 40 x 33 cm (l x d x h)

Weight

12 Kg

AutoQuest Autosampler  4800-100

Cecil Instruments policy is one of continuous development. We therefore reserve the right to change specifications without notice.

 
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